Publications

  • Macrophage-pathogen interactions in infectious diseases

    Macrophage-pathogen interactions in infectious diseases

    This review discusses how several bacterial and fungal infection models in zebrafish embryos have led to new insights into the dynamic molecular and cellular mechanisms at play when pathogens encounter host macrophages. It also describes how these insights are inspiring novel therapeutic strategies for infectious disease treatment.

    Torraca V, Masud S, Spaink HP, Meijer AH. Dis Model Mech. 2014 Jul;7(7):785-97.

    Read the paper

  • In Vivo Assessment of Drug Efficacy against Mycobacterium abscessus

    In Vivo Assessment of Drug Efficacy against Mycobacterium abscessus

    This paper presents a new and efficient method for testing and compare the in vivo activity of two clinically relevant drugs based on a fluorescent reporter strain in zebrafish embryos. This approach could be used for rapid determination of the in vivo drug susceptibility profile of clinical isolates and to assess the preclinical efficacy of new compounds against M. abscessus.

    Bernut A, Le Moigne V, Lesne T, Lutfalla G, Herrmann JL, Kremer L. Antimicrob Agents Chemother. 2014 Jul;58(7):4054-4063. Epub 2014 May 5.

    Read the paper

  • Dual role for complement in the inflammation/regeneration processes in zebrafish

    Dual role for complement in the inflammation/regeneration processes in zebrafish

    In addition to the description to c3 genes in the zebrafish, this paper most importantly shows the differential abilities of C3 paralogs to regulate cytokine production and neutrophil migration upon injury, indicating a dual role for complement in the inflammation/regeneration processes in zebrafish

    Forn-Cuní G, Reis ES, Dios S, Posada D, Lambris JD, Figueras A, Novoa B. PLoS One. 2014 Jun 13;9(6):e99673. doi: 10.1371/journal.pone.0099673.

    Read the paper

  • Clonal Expansion during Staphylococcus aureus Infection Dynamics Reveals the Effect of Antibiotic Intervention

    Clonal Expansion during Staphylococcus aureus Infection Dynamics Reveals the Effect of Antibiotic Intervention

    Authors demonstrate results using mouse and zebrafish systemic infection models to study intervention with sub-curative antibiotic doses. These doses are able to support the preferential expansion of antibiotic-resistant organisms due to the clonal pattern of infection or on the spontaneous generation of resistance. These results have important implications for the design of properly-controlled treatment regimes.

    McVicker G, Prajsnar TK, Williams A, Wagner NL, Boots M, Renshaw SA, Foster SJ. PLoS Pathog. 2014 Feb 27;10(2):e1003959. doi: 10.1371/journal.ppat.1003959.

    Click here for the paper

  • Cording in the in vivo physiopathology of M. abscessus infection

    Cording in the in vivo physiopathology of M. abscessus infection

    The authors demonstrate that extracellular cording allows the bacterium to withstand phagocytosis, leading to uncontrolled growth and establishment of an acute and lethal infection, thus constituting a determinant of virulence.

    Bernut A, Herrmann JL, Kissa K, Dubremetz JF, Gaillard JL, Lutfalla G, Kremer L. Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):E943-52. doi: 10.1073/pnas.1321390111.

    Read the paper

  • High-throughput pipeline to study S. epidermidis and M. marinum infection

    High-throughput pipeline to study S. epidermidis and M. marinum infection

    The described new high-throughput pipeline allows the generation and analysis of large number of synchronous embryos homogenously infected. Moreover the flexibility of the pipeline allows the user to easily implement other platforms to improve the resolution of the analysis when needed.

    Veneman WJ, Marín-Juez R, de Sonneville J, Ordas A, Jong-Raadsen S, Meijer AH, Spaink HP. J Vis Exp. 2014 Jun 26;(88):e51649. doi: 10.3791/51649.

    Watch the video

  • A tentative model for the role of Map9 in zebrafish development

    A tentative model for the role of Map9 in zebrafish development

    In vivo results of this publication confirm the previous in vitro observations about ASAP/MAP9’s role in regulating MT dynamics, and suggest that Map9 is a key factor during the early steps of zebrafish development.

    Fontenille L, Rouquier S, Lutfalla G, Giorgi D. Cell Cycle. 2014 Apr 1;13(7):1101-14. doi: 10.4161/cc.27944. Epub 2014 Feb 4.

    Read the paper here

  • Addressing major questions of host–virus interactions with zebrafish model

    Addressing major questions of host–virus interactions with zebrafish model

    This article discusses the pros and cons of zebrafish as a model for host–virus interactions, and addresses the spread of the viral infection within the body as well as the tolerance versus resistance concept in zebrafish.

    Levraud JP, Palha N, Langevin C, Boudinot P. Trends Microbiol. 2014 May 24. pii: S0966-842X(14)00096-1. doi: 10.1016/j.tim.2014.04.014.

    Read the publication here

  • DRAM1 Links Mycobacterial Recognition via TLR-MYD88 to Autophagic Defense

    DRAM1 Links Mycobacterial Recognition via TLR-MYD88 to Autophagic Defense

    Exploiting the advantages of the zebrafish in vivo model for tuberculosis, van der Vaart et al. discovered that DRAM1 is a protein that regulates anti-bacterial autophagy. DRAM1 cooperates with the selective autophagy receptor p62 and promotes the fusion of autophagosomes with lysosomes where mycobacteria are killed.

    van der Vaart M, Korbee CJ, Lamers GE, Tengeler AC, Hosseini R, Haks MC, Ottenhoff TH, Spaink HP, Meijer AH. Cell Host Microbe. 2014 Jun 11;15(6):753-67. doi: 10.1016/j.chom.2014.05.005.

    Read the publication here

  • Tnfa Signaling Through Tnfr2 Protects Skin Against Oxidative Stress–Induced Inflammation

    Tnfa Signaling Through Tnfr2 Protects Skin Against Oxidative Stress–Induced Inflammation

    This paper discusses the identification of a crucial role for TNFa/TNFR2 axis in the protection of the skin against DUOX1-mediated oxidative stress and that these results could establish new therapeutic targets for skin inflammatory disorders.

    Candel S, de Oliveira S, López-Muñoz A, García-Moreno D, Espín-Palazón R, Tyrkalska SD, Cayuela ML, Renshaw SA, Corbalán-Vélez R, Vidal-Abarca I, Tsai HJ, Meseguer J, Sepulcre MP, Mulero V. PLoS Biol. 2014 May 6;12(5):e1001855. doi: 10.1371/journal.pbio.1001855. eCollection 2014.

    Read the paper here

European funding supports the training of young scientists in the FishForPharma network